A fast and safe bisulfite sequencing analysis platform
BiSuflite Bolt (BSBolt); a fast and scalable bisulfite sequencing analysis platform. BSBolt is an integrated analysis platform that offers support for bisulfite sequencing read simulation, alignment, methylation calling, data aggregation, and data imputation. BSBolt has been validated to work with a wide array of bisulfite sequencing data,including whole genome bisulfite sequencing (WGBS), reduced representative bisulfite sequencing data (RRBS), and targeted methylation sequencing data. BSBolt utilizes forked versions of BWA and WGSIM for read alignment and read simulation respectively. BSBolt is released under the MIT license.
Farrell, C., Thompson, M., Tosevska, A., Oyetunde, A. & Pellegrini, M. BiSulfite Bolt: A BiSulfite Sequencing Analysis Platform. 2020.10.06.328559 (2020). doi:10.1101/2020.10.06.328559
- Fixed bug ending alignment when the reference template end greater than reference boundary.
- Alignment stats fix.
- Alignment statistics now output as generated.
- Fixed bug where alignment would stop when observed mappability was low.
- Fixed maximum read depth bug that prevented methylation call on site covered by greater than 8000 reads
- Refactored build script, with experimental support for M1 Macs
- The default entry point for BSBolt has changed from BSBolt to bsbolt for conda compatibility
Pre-compiled binaries can be installed using PyPi. Binaries are available for python >=3.6 on unix like systems (macOS >=10.15 and linux).
pip3 install bsbolt --user
BSBolt can be installed using the conda package manager using the instructions below.
conda config --add channels bioconda conda config --add channels conda-forge conda install -c cpfarrell bsbolt
Installing from Source
- zlib-devel >= 1.2.3-29
- GCC >= 8.3.1
# clone the repository git clone https://github.com/NuttyLogic/BSBolt.git cd bsbolt # compile and install package pip3 install .
Installing from Source on macOS
Installation from source requires xcode command line utilities, homebrew macOS package manager, autoconf, python (>=3.6), and automake.The full installation process is outlined below.
# install xcode utilities xcode-select --install # install homebrew /bin/bash -c "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/HEAD/install.sh)" # install autoconf brew install autoconf # install automake brew installa automake # optionally install python > 3.5 brew install python3.8 # clone the repository git clone https://github.com/NuttyLogic/BSBolt.git cd bsbolt # compile and install package pip3 install .
Following installation BSBolt can be called using bsbolt Module.
python3 -m bsbolt
bsbolt Module Align Alignment Index Index Generation CallMethylation Methylation Calling AggregateMatrix CGmap Matrix Aggregation Simulate bsbolt Illumina Read Simulation Impute kNN Imputation Sort Sort BAM File BamIndex Index BAM file